blog – journal club

Here we list interesting new papers, excellent material for our monthly journal club:

July 2018: many different papers report the identification of the Shieldin protein complex: Specifically, C20orf196 ( SHLD1), FAM35A (SHLD2) and CTC-534A2.2 (SHLD3) were shown to work downstream of 53Bp1-Rif1-PTIP-REV7 in DNA break repair. Nice work by the Durocher lab (link), de Lange lab (link), Jackson lab (link) and choudhary lab (link). update: an additional paper by the Chapman lab reports a role for the shielding complex in  NHEJ and VDJ re-arrangement (link)

May 2018: interesting paper by the Pasaro lab in Nature, showing the cytoplasmic DNA can originate from stalled replication forks, and that SAMHD1 prevent this (link)

November 2017: Many interesting papers have come out on replication fork stability in HR-deficient cells. Comprehensive editorial by Schlacher in Nature Cell Biology (link), and two papers in Nature Communications form the labs of Lopes (link) and Jasin (link)

July 2017: cool paper on Abro1 in Genes and Development (link).

November 2015: Three papers, two in Science and one in Cell, describe which genes are essential for viability in human cells. The labs of Brummelkamp (link) and Sabatini/Lander (link) use intsertional mutagenesis  in haploid cells and CRISPR/Cas9-mediated gene mutation in non-haploid human cancer cell lines to identify a list of ~2,000 essential genes. The Moffat lab (link) also used CRISPR/Cas9 in a larger set of cancer cell lines, and also analysed non-cancer cell lines to identify a similar list of essential genes, but also identify specific genes that are only essential selected cancer cell lines, based on their specific genetic make-up.

November 2015: The lab of Titia de Lange published a paper in Cell (link), which describes the motility of a DNA break, induced by telomere dysfunction or irradiation. DSB mobility promotes NHEJ repair, and is detrimental in situations of defective HR repair.

September 2015: two new papers in Molecular Cell uncover molecular requirements to counter replication stress in S Phase. Work from the Zou lab (link) showed that ATR control origin firing through RRM2 accumulation in a subset of cells. In other S phase cells, however, ATR triggers DNA-PK and Chk1 signalling to block origin firing. Work from the Cortex lab (link) uses iPOND mass spec technology to map configurations of unperturbed replisomes versus replisomes of stress cells, and cells in which ATR is inhibited.

September 2015: Steve Ellegde and Evelyn Witkin were awarded the Albert Lasker Award for Basic Medical Research. In Cell, the contributions of these pioneers are discussed by James Haber (link to website), and two entertaining stories by Elledge (link to website) and Witkin (link to website) are featured.